Difference between revisions of "Part:BBa K3468070"
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<partinfo>BBa_K3468070 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468070 SequenceAndFeatures</partinfo> | ||
Adding hydrogen bonds | Adding hydrogen bonds | ||
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| + | The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:T270Q.png|400px|thumb|left|Fig.1 T270Q in PyMOL]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 13:57, 27 October 2020
PETase T270Q
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to Q at 270 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Adding hydrogen bonds
The reduction of DDG was predicted by FoldX, and PyOML was used for visual screening. it formed hydrogen bonds with 273-CYS, 274-GLU and 291-LEU, which was mutated into GLN, which was beneficial to improve the thermal stability of PETase.
