Difference between revisions of "Part:BBa K3429002"
 
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<partinfo>BBa_K3429002 short</partinfo>
 
<partinfo>BBa_K3429002 short</partinfo>
  
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Flourescence Protein optimized for B. subtilis codon usage.
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<h2>Profile</h2>
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<br>
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            <table style=“width:80%“>
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We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA and CuO. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function.
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                <tr>
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                    <td><b>Name</b></td>
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                    <td>superfolder Green Fluorescent Protein (sfGFP)</td>
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                </tr>
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                <tr>
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                    <td><b>Base pairs</b></td>
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                    <td>714</td>
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                </tr>
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                <tr>
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                    <td><b>Molecular weight</b></td>
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                    <td>26.8&nbsp;kDa</td>
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                </tr>
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                <tr>
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                    <td><b>Origin</b></td>
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                    <td><i>Aequorea victoria</i>, synthetic</td>
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                </tr>
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            </table>
  
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<br> <br>
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The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein<sup>[1]</sup>.  We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.
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<h2>References</h2>
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[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2
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<a class="anchor" id="cite_note-1"></a>
 
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            <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>
 
  
  

Latest revision as of 09:29, 26 October 2020


Superfolder Green Fluorescent Protein (sfGFP)

Profile

Name superfolder Green Fluorescent Protein (sfGFP)
Base pairs 714
Molecular weight 26.8 kDa
Origin Aequorea victoria, synthetic

The superfolder green fluorescent protein (sfGFP) is a, for B. subtilis codon-optimized, derivative of the GFP. Huang et al. was able to demonstrate its function as a reporter fused with the TasA protein[1]. We implement it as a reporter and experimental control for our TasA fusion proteins with the enzymes CotA, CuO and EreB.


References

[1] Huang, J., Liu, S., Zhang, C. et al., Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41, (2019), https://doi.org/10.1038/s41589-018-0169-2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]