Difference between revisions of "Part:BBa K3466003"

Latest revision as of 22:12, 24 October 2020

CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT

This plasmid is an alternate version of part BBa_K3466000 with the addition of BamHI and BgIII restriction sites surrounding the RBS. These restriction sites allow us to cut and insert the single RFP part (BBa_K3466006) into the plasmid. This is because in our previous experiment, having more RFP genes may have increased the intensity and the rate of expression of RFP. Therefore, we want to test whether more RFP genes lead to better RFP expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 293
Illegal AgeI site found at 395
Illegal AgeI site found at 1168
Illegal AgeI site found at 1280
1000
COMPATIBLE WITH RFC[1000]

Revision as of 22:08, 24 October 2020 (view source) Registry (Talk \| contribs)		Latest revision as of 22:12, 24 October 2020 (view source) Isabelagonzalez (Talk \| contribs)
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	<partinfo>BBa_K3466003 short</partinfo>		<partinfo>BBa_K3466003 short</partinfo>

−	This plasmid is an alternate version of ~~construct 1~~ with the addition of BamHI and BgIII restriction sites surrounding the RBS. These restriction sites allow us to cut and insert the single RFP part (BBa_K3466006) into the plasmid. This is because in our previous experiment, having more RFP genes may have increased the intensity and the rate of expression of RFP. Therefore, we want to test whether more RFP genes lead to better RFP expression.	+	This plasmid is an alternate version of part BBa_K3466000 with the addition of BamHI and BgIII restriction sites surrounding the RBS. These restriction sites allow us to cut and insert the single RFP part (BBa_K3466006) into the plasmid. This is because in our previous experiment, having more RFP genes may have increased the intensity and the rate of expression of RFP. Therefore, we want to test whether more RFP genes lead to better RFP expression.