Difference between revisions of "Part:BBa K3466001:Design"
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===Source=== | ===Source=== | ||
| − | Constitutive promoter - BBa_J23100 | + | Constitutive promoter - BBa_J23100, RBS+MerR - BBa_K1724002, PcadA promoter - BBa_K1724000, RBS - BBa_J34801, mRFP - BBa_I13521, TT - BBa_B0015 |
| − | RBS+MerR - BBa_K1724002 | + | |
| − | PcadA promoter - BBa_K1724000 | + | |
| − | RBS - BBa_J34801 | + | |
| − | mRFP - BBa_I13521 | + | |
| − | TT - BBa_B0015 | + | |
===References=== | ===References=== | ||
Latest revision as of 21:59, 24 October 2020
CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 293
Illegal AgeI site found at 395
Illegal AgeI site found at 1168
Illegal AgeI site found at 1280 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a spacer to test whether or not it serves to enhance our RFP expression rate.
Source
Constitutive promoter - BBa_J23100, RBS+MerR - BBa_K1724002, PcadA promoter - BBa_K1724000, RBS - BBa_J34801, mRFP - BBa_I13521, TT - BBa_B0015