Difference between revisions of "Part:BBa K3332078"
 
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<partinfo>BBa_K3332078 short</partinfo>
 
<partinfo>BBa_K3332078 short</partinfo>
  
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It can express toxin protein according to the concentration of formaldehyde.It ensures that our engineering bacteria die when the concentration of glyphosate is low to a certain level.
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It can express toxin protein if there is no formaldehyde.
  
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===Usage and Biology===
 
===Usage and Biology===
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Glyphosate can be metabolized into formaldehyde in ''E.coli''. Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter(<partinfo>BBa_Q04510</partinfo>) plays the role of gene inversion which reverses expression pattern when existing. MazF(<partinfo>BBa_K1720002</partinfo>) was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity.
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<html>Glyphosate is metabolized into formaldehyde in the body of bacteria
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    <figure>
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        <img src="https://2020.igem.org/wiki/images/9/9b/T--XMU-China--XMU-China_2020-pHCHO_inverter_mazF_B0015.png" width="60%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
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'''Fig 1.''' pHCHO_inverter_mazF_B0015
  
 
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Latest revision as of 22:47, 27 October 2020


Formaldehyde promoter-Inverter-MazF-terminator

It can express toxin protein if there is no formaldehyde.

Usage and Biology

Glyphosate can be metabolized into formaldehyde in E.coli. Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter(BBa_Q04510) plays the role of gene inversion which reverses expression pattern when existing. MazF(BBa_K1720002) was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity.

Glyphosate is metabolized into formaldehyde in the body of bacteria

Fig 1. pHCHO_inverter_mazF_B0015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]