Difference between revisions of "Part:BBa K3332086"

Latest revision as of 20:53, 27 October 2020

pTrc-2-RBS-ECFP-terminator

With this part, the pTrc-2 promoter can be tested by observing the fluorescence intensity/OD.

Usage and Biology

This part can be used to test that if the pTrc-2 promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 promoter.

Fig 1. pTrc-2_RBS(B0034)_ECFP(E0020)_terminator(B0015)

Characterization

The agarose gel electrophoresis images are below:

Fig 2. pTrc-2_E0420_pSB1C3 digested by Xba I and Pst I(about 1003bp)

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3332086 short</partinfo>
-We use pTrc-2 promoter to express ECFP.
+With this part, the pTrc-2 promoter can be tested by observing the fluorescence intensity/OD.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part can be used to test that if the pTrc-2 promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 promoter.
-<!-- -->
+<html>
-<span class='h3bb'>Sequence and Features</span>
+    <figure>
-<partinfo>BBa_K3332086 SequenceAndFeatures</partinfo>
+        <img src="https://2020.igem.org/wiki/images/d/d1/T--XMU-China--XMU-China_2020-pTrc-2_B0034_ecfp_B0015.png" width="40%" style="float:center">
+        <figcaption>
+        <p style="font-size:1rem">
+        </p>
+        </figcaption>
+    </figure>
+</html>
+'''Fig 1.''' pTrc-2_RBS(B0034)_ECFP(E0020)_terminator(B0015)
+===Characterization===
+The agarose gel electrophoresis images are below:
+[[File:T--XMU-CHINA-BBa K3332086.png|none|600px|caption]]
+'''Fig 2. '''pTrc-2_E0420_pSB1C3 digested by ''Xba ''I and ''Pst'' I(about 1003bp)
+===Reference===
+[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. &apos;Deadman&apos; and &apos;Passcode&apos; microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979
 <!-- Uncomment this to enable Functional Parameter display
@@ Line 17: / Line 30: @@
 <partinfo>BBa_K3332086 parameters</partinfo>
 <!-- -->
+===Sequence and Features===
+<partinfo>BBa_K3332086 SequenceAndFeatures</partinfo>