Difference between revisions of "Part:BBa K3589151"
(One intermediate revision by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K3589151 short</partinfo> | <partinfo>BBa_K3589151 short</partinfo> | ||
− | This basic part contains the sequence of | + | <html> |
+ | <p> | ||
+ | This basic part contains the sequence of the HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like <a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a> (cCA (B2)), <a href="https://parts.igem.org/Part:BBa_K3002008">BBa_K3002008</a> (GLE (B2)) or <a href="https://parts.igem.org/Part:BBa_K3002009">BBa_3002009</a> (ARS (B2)), an appropriate promoter (<a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001 </a>(PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein should be secreted and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.<br> | ||
+ | Note that this part misses a SP20-portion. The SP20-portion is reported to improve the secretion of proteins by increasing the glycosylation (see Team TU Kaiserslautern 2019's <a href="https://2019.igem.org/Team:TU_Kaiserslautern/Results">results</a>). It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (<a href="https://doi.org/10.1111/pbi.12710">Ramos-Martinez <I>et. al</I>, 2017</a>).<br><br> | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3589150">BBa_K3589150</a> is a variation of this tag which possesses the SP20-portion. | ||
+ | </p> | ||
+ | <br><br> | ||
+ | <p> | ||
+ | <b>Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589151</b><br> | ||
+ | <ul type=“square“> | ||
+ | <li>We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <br> | ||
+ | For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:<br> | ||
+ | <p> | ||
+ | <u>L1</u><br> | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3589205">BBa_K3589205</a><br> | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3589206">BBa_K3589206</a><br> | ||
+ | <br> | ||
+ | </p> | ||
+ | <P> | ||
+ | <u>L2</u><br> | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3589211">BBa_K3589211</a><br> | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3589212">BBa_K3589212</a><br> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 19:15, 27 October 2020
HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the sequence of the HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein should be secreted and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
Note that this part misses a SP20-portion. The SP20-portion is reported to improve the secretion of proteins by increasing the glycosylation (see Team TU Kaiserslautern 2019's results). It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (Ramos-Martinez et. al, 2017).
BBa_K3589150 is a variation of this tag which possesses the SP20-portion.
Summary of the Results from Team Kaiserslautern 2020 for part BBa_K3589151
- We could not detect our secreted protein of interest when combined with this tag, as well as a cCA secretion signal.
For more information, please visit the registry pages for the L1 and L2 constructs containing this tag:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]