Difference between revisions of "Part:BBa K3599035"

Latest revision as of 14:41, 27 October 2020

baPAD

an enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of baPAD was converting 4-coumaric acid into 4-hydroxystyrene. The codon usage was optimized for expression in E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 220
1000
COMPATIBLE WITH RFC[1000]

Reference

Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. Microbial cell factories, 14(1), 1-11. 10.1186/s12934-015-0268-7

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3599035 short</partinfo>
-an enzyme which catalyze the artificial biosynthesis pathway to produce hydroxystyrene
+an enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of baPAD was converting 4-coumaric acid into 4-hydroxystyrene. The codon usage was optimized for expression in <i>E. coli</i>.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
+===Sequence and Features===
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3599035 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
+===Reference===
-===Functional Parameters===
+Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. <i>Microbial cell factories</i>, 14(1), 1-11. [https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0268-7 10.1186/s12934-015-0268-7]
-<partinfo>BBa_K3599035 parameters</partinfo>
-<!-- -->