Difference between revisions of "Part:BBa K3580102:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in the2015 paper by Srividya, Narayanan et al.The one-amino acid mutation was introduced into the limonene synthase of Mentha spicatato accommodate the N345A mutation, as reported in a 2015 paper by Srividya and Narayanan et al.Actually, limonene synthase used in our project was an E. colicodon-optimized version of a truncated sequence, so a mutation was introduced to change asparagine at position 289 to alanine. | |
| + | ===Source=== | ||
| + | The part BBa_K3580102 is a new part for Parts Registry. It can synthesize sabinene and be used in combination with pBbA5c-MevT-MBI. One amino acid mutation was introduced into limonene synthase of Mentha spicata to create sabinene synthase, by using information reported in a 2015 paper by Srividya and Narayanan et al. | ||
| + | ===References=== | ||
| + | (1) Alonso-Gutierrez, J. et al. (2013). Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metabolic engineering, 19, 33-41. | ||
| − | + | (2) Dudley, Q. M. et al. (2019). Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates. Synthetic Biology, 4(1), ysz003. | |
| − | + | (3) Srividya, N. et al. (2015). Functional analysis of (4S)-limonene synthase mutants reveals determinants of catalytic outcome in a model monoterpene synthase. Proceedings of the National Academy of Sciences, 112(11), 3332-3337. | |
| − | + | (4) Korman, T. P. et al. (2017). A synthetic biochemistry platform for cell free production of monoterpenes from glucose. Nature communications, 8(1), 1-8. | |
| + | |||
| + | (5) Mass Spectrometry Data Center, William E. Wallace, "Mass Spectra" in NIST Chemistry WebBook, NIST Standard Reference Database Number 69, Eds. P.J. Linstrom and W.G. Mallard, National Institute of Standards and Technology, Gaithersburg MD, 20899, https://doi.org/10.18434/T4D303, (retrieved October 22, 2020). | ||
Latest revision as of 13:33, 27 October 2020
Status: 500
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Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in the2015 paper by Srividya, Narayanan et al.The one-amino acid mutation was introduced into the limonene synthase of Mentha spicatato accommodate the N345A mutation, as reported in a 2015 paper by Srividya and Narayanan et al.Actually, limonene synthase used in our project was an E. colicodon-optimized version of a truncated sequence, so a mutation was introduced to change asparagine at position 289 to alanine.
Source
The part BBa_K3580102 is a new part for Parts Registry. It can synthesize sabinene and be used in combination with pBbA5c-MevT-MBI. One amino acid mutation was introduced into limonene synthase of Mentha spicata to create sabinene synthase, by using information reported in a 2015 paper by Srividya and Narayanan et al.
References
(1) Alonso-Gutierrez, J. et al. (2013). Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metabolic engineering, 19, 33-41.
(2) Dudley, Q. M. et al. (2019). Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates. Synthetic Biology, 4(1), ysz003.
(3) Srividya, N. et al. (2015). Functional analysis of (4S)-limonene synthase mutants reveals determinants of catalytic outcome in a model monoterpene synthase. Proceedings of the National Academy of Sciences, 112(11), 3332-3337.
(4) Korman, T. P. et al. (2017). A synthetic biochemistry platform for cell free production of monoterpenes from glucose. Nature communications, 8(1), 1-8.
(5) Mass Spectrometry Data Center, William E. Wallace, "Mass Spectra" in NIST Chemistry WebBook, NIST Standard Reference Database Number 69, Eds. P.J. Linstrom and W.G. Mallard, National Institute of Standards and Technology, Gaithersburg MD, 20899, https://doi.org/10.18434/T4D303, (retrieved October 22, 2020).