Difference between revisions of "Part:BBa K133017:Design"

 
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<partinfo>BBa_K133017 short</partinfo>
 
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The CF-multiHP-RGD-Histop was cloned into pSB1.AK3 without terminator for use in bacterial cells.
  
 
===Source===
 
===Source===
  
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plasmid
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plasmid: Red Flourescence Protein was obtaind from previousely designed biobrick of 2007 iGEM team Ljubljana containing Red Flourescence Protein with added nuclear localisation signal and mutated PstI restriction site.
  
 
===References===
 
===References===

Latest revision as of 01:52, 30 October 2008

mCherry-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


Design Notes

none

The CF-multiHP-RGD-Histop was cloned into pSB1.AK3 without terminator for use in bacterial cells.

Source

plasmid: Red Flourescence Protein was obtaind from previousely designed biobrick of 2007 iGEM team Ljubljana containing Red Flourescence Protein with added nuclear localisation signal and mutated PstI restriction site.

References