Difference between revisions of "Part:BBa K3360002:Design"

Latest revision as of 22:04, 23 October 2020

Thermostable beta-glucosidase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1577
Illegal AgeI site found at 927
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

Source

Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons

References

Zhang Hui, Qiu Riyong, Liu Mingjie, et al. Cloning and overexpression of extremely heat-resistant β-glucosidase gene and its application in the industrial production of natural blue pigment[J].Jiangsu Agricultural Sciences,2013,41(7 ):19-22.

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	===References===		===References===
		+	Zhang Hui, Qiu Riyong, Liu Mingjie, et al. Cloning and overexpression of extremely heat-resistant β-glucosidase gene and its application in the industrial production of natural blue pigment[J].Jiangsu Agricultural Sciences,2013,41(7 ):19-22.