Difference between revisions of "Part:BBa K3450012"
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K3450012 short</partinfo>
 +
 
This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor.
 
This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor.
 
This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin.
 
This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin.
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K3450012 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K3450012 parameters</partinfo>
 +
<!-- -->

Latest revision as of 15:12, 26 October 2020

Phosphate-Sensitive Kill Switch: Allows E. coli to express E2-IM2 toxin-antitoxin system sensor

This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor. This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 464
    Illegal NheI site found at 487
    Illegal NheI site found at 1825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 217
    Illegal BglII site found at 1394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]