Difference between revisions of "Part:BBa K3606087"

Latest revision as of 00:56, 28 October 2020

PlacIq_lacI_Ptac driven mcbEFG

This is a plamid with high expression level.This part works as an adjusting part in quorum sensing system. When the number of engineered bacteria is low, the part will help export antibiotic and provide self immunity in order to help proliferate.

Background:

Here, we tried to improve the former antimicrobial peptide(mccb17) expressing system of 2019 Fudan BBa_K3245010. By dividing into the peptide expressing parts and the immunity parts, we wanted to firstly test whether the polycistron could work properly and separately, then manipulate their expression level with more efficiency.

Design:

This part is a immunity system coding gene cluster with efflux and immunity related protein.

Usage and Biology:

This part works as an adjusting part in quorum sensing system. When the number of engineered bacteria is low, the part will help export antibiotic and provide self immunity in order to help proliferate.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3606087 short</partinfo>
-tba
+This is a plamid with high expression level.This part works as an adjusting part in quorum sensing system. When the number of engineered bacteria is low, the part will help export antibiotic and provide self immunity in order to help proliferate.
+<h2>Background:</h2>
+Here, we tried to improve the former antimicrobial peptide(mccb17) expressing system of 2019 Fudan [[BBa_K3245010]]. By dividing into the peptide expressing parts and the immunity parts, we wanted to firstly test whether the polycistron could work properly and separately, then
+manipulate their expression level with more efficiency.
+<h2>Design:</h2>
+This part is a immunity system coding gene cluster with efflux and immunity related protein.
+[[File: T--Fudan--img_mcbefg.png|none|600px|thumb]]
+<h2>Usage and Biology:</h2>
+This part works as an adjusting part in quorum sensing system. When the number of engineered bacteria is low, the part will help export antibiotic and provide self immunity in order to help proliferate.
 <!-- Add more about the biology of this part here