Difference between revisions of "Part:BBa K3338014"

 
 
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This part contains an EGFP-IRES-mCherry cassette under control of the constitutively active CMV-promoter. The fluorescent proteins EGFP and mCherry can be independently detected. It was used to compare the functionality of an IRES and the P2A peptide for the expression of two proteins from one single promoter.
 
This part contains an EGFP-IRES-mCherry cassette under control of the constitutively active CMV-promoter. The fluorescent proteins EGFP and mCherry can be independently detected. It was used to compare the functionality of an IRES and the P2A peptide for the expression of two proteins from one single promoter.
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K3338014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3338014 SequenceAndFeatures</partinfo>
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=Characterization=
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This part was used to characterize the IRES. A detailed documentation can be found on the page of the basic part <html><a href=" https://parts.igem.org/Part:BBa_K3338004">BBa_K3338004</a></html>.
  
  

Latest revision as of 00:35, 27 October 2020


CMV-EGFP-IRES-mCherry

This part contains an EGFP-IRES-mCherry cassette under control of the constitutively active CMV-promoter. The fluorescent proteins EGFP and mCherry can be independently detected. It was used to compare the functionality of an IRES and the P2A peptide for the expression of two proteins from one single promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This part was used to characterize the IRES. A detailed documentation can be found on the page of the basic part BBa_K3338004.