Difference between revisions of "Part:BBa K3482011"
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| − | The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator. | + | The pTac promoter sequence here is preceded by a binding region (operator) for the repressor <i>LacI</i>; the binding of <i>LacI</i> prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to <i>LacI</i> and releases it from its operator. |
| − | We use this pTac promoter in our kill | + | We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG. |
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Latest revision as of 19:22, 27 October 2020
pTac promoter
The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.
We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]