Difference between revisions of "Part:BBa K3606041"
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<partinfo>BBa_K3606041 short</partinfo> | <partinfo>BBa_K3606041 short</partinfo> | ||
− | + | PhoA_MBP is constructed for the test and verification of secretion led by PhoA. | |
− | <!-- | + | <!-- --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <p>MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli. </p> | ||
+ | <p>To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.</p> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th> Signal Peptide </th> <th> Number for Registry </th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> NSP4 </td> <td> BBa_K3606042 </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> OmpA </td> <td> BBa_K3606043 </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> DsbA </td> <td> BBa_K3606030 </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> PelB </td> <td> BBa_K208004 </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> PhoA </td> <td> BBa_K808028 </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | <p>Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.</p> | ||
+ | |||
+ | <p>To test the secretion efficiency of our 5 secretion peptides and compare their secretion capabilities, we also fused them to MBP(Maltose Binding Protein) that normally expressed in the cytoplasm of E.coli. cells. Driven by an iPTG inducible promoter Ptac, we can achieve quantified characterization of these secretion peptides.</p> | ||
<!-- --> | <!-- --> | ||
− | < | + | <h4>Sequence and Features</h4> |
<partinfo>BBa_K3606041 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3606041 SequenceAndFeatures</partinfo> | ||
Latest revision as of 12:55, 26 October 2020
PlacIq_lacI_Ptac driven PhoA-MBP
PhoA_MBP is constructed for the test and verification of secretion led by PhoA.
Usage and Biology
MBP(Maltose Binding Protein) is a protein that normally expressed in the cytoplasm of E.coli.
To test the efficiency of our 5 signal peptides for the secretion of the target protein, we fused our 5 signal peptides to MBP to compare their secretion capabilities. The signal peptides and corresponding serial numbers are listed below, through which the biology and characterization of each peptide are available.
Signal Peptide | Number for Registry |
---|---|
NSP4 | BBa_K3606042 |
OmpA | BBa_K3606043 |
DsbA | BBa_K3606030 |
PelB | BBa_K208004 |
PhoA | BBa_K808028 |
Moreover, we put the expression of secretion peptide guided MBP under the regulation of Ptac, an iPTG inducible promoter, to achieve better qualification of our secretion peptides.
To test the secretion efficiency of our 5 secretion peptides and compare their secretion capabilities, we also fused them to MBP(Maltose Binding Protein) that normally expressed in the cytoplasm of E.coli. cells. Driven by an iPTG inducible promoter Ptac, we can achieve quantified characterization of these secretion peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1430