Difference between revisions of "Part:BBa K3561028"

Latest revision as of 15:45, 23 October 2020

TSNAVAWTLRCL-CBD

This fusion is a fusion of our palladium reducing peptide and cellulose binding domain. The palladium reducing peptide is expected to reduce palladium and the cellulose binding domain is expected to adsorb to cellulose. More details of the palladium reducing peptide can be found in part BBa_K3561013, this is one of the best performing peptides. The sequence of the cellulose binding domain is provided by Prod. Shen Long Tsai and can be found in part BBa_K3561026.

We have added a NheI and a PStI cut site at the start and end of the sequence respectively. An EcoRI cut site is added in between the peptide and the CBD. The codons are also optimised.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 37
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 37
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 37
Illegal AgeI site found at 145
Illegal AgeI site found at 355
1000
COMPATIBLE WITH RFC[1000]

References

Yunus, Ian Sofian, and Shen-Long Tsai. “Designed Biomolecule–Cellulose Complexes for Palladium Recovery and Detoxification.” RSC Advances, The Royal Society of Chemistry, 12 Feb. 2015, pubs.rsc.org/en/content/articlelanding/2015/ra/c4ra16200e.

@@ Line 13: / Line 13: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3561028 SequenceAndFeatures</partinfo>
+<h2>References</h2>
+Yunus, Ian Sofian, and Shen-Long Tsai. “Designed Biomolecule–Cellulose Complexes for Palladium Recovery and Detoxification.” RSC Advances, The Royal Society of Chemistry, 12 Feb. 2015, pubs.rsc.org/en/content/articlelanding/2015/ra/c4ra16200e.