Difference between revisions of "Part:BBa K3580002"
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<partinfo>BBa_K3580002 short</partinfo> | <partinfo>BBa_K3580002 short</partinfo> | ||
− | This is an improvement of BBa_B0015. Instead of GFP(BBa_E0040), we use our new parts: GFPssrA(BBa_K3580001). | + | This is an improvement of BBa_B0015. Instead of GFP([https://parts.igem.org/Part:BBa_E0040 BBa_E0040]), we use our new parts: GFPssrA([https://parts.igem.org/Part:BBa_K3580001 BBa_K3580001]). |
− | By adding this parts understream of a promoter, it can express GFP with degradation tag. | + | By adding this parts understream of a promoter, it can express GFP with a degradation tag. |
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Latest revision as of 22:52, 27 October 2020
GFPssrA with RBS and double terminator
This is an improvement of BBa_B0015. Instead of GFP(BBa_E0040), we use our new parts: GFPssrA(BBa_K3580001). By adding this parts understream of a promoter, it can express GFP with a degradation tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 656