Difference between revisions of "Part:BBa K3365013"
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<partinfo>BBa_K3365013 short</partinfo> | <partinfo>BBa_K3365013 short</partinfo> | ||
| − | + | It contains the pBAD promoter of the arabinose operon (BBa_K3365009) and its regulatory gene, <i>araC</i> (BBa_K2442103). The AraC protein is both a positive and a negative regulator. In the presence of arabinose, transcription from the pBAD promoter is turned on; in its absence, transcription occurs at very low levels. The uninduced levels can be reduced even further by growth in the presence of glucose. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The regulatory can be used to achieve very low levels of expression in the presence of inducer, obtain moderately high levels of expression in the presence of inducer, and modulate expression over a wide range of inducer concentrations. | ||
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Latest revision as of 13:56, 27 October 2020
Inducible pBAD/araC promoter
It contains the pBAD promoter of the arabinose operon (BBa_K3365009) and its regulatory gene, araC (BBa_K2442103). The AraC protein is both a positive and a negative regulator. In the presence of arabinose, transcription from the pBAD promoter is turned on; in its absence, transcription occurs at very low levels. The uninduced levels can be reduced even further by growth in the presence of glucose.
Usage and Biology
The regulatory can be used to achieve very low levels of expression in the presence of inducer, obtain moderately high levels of expression in the presence of inducer, and modulate expression over a wide range of inducer concentrations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961