Difference between revisions of "Part:BBa K3425105"

 
 
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<partinfo>BBa_K3425105 short</partinfo>
 
<partinfo>BBa_K3425105 short</partinfo>
  
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Device expressing BBa_K3425100 in backbone pSB3C11 (BBa_K3425001) with promoter J23108 and B0034.  
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BBa K3425105 is a device expressing the DNA Binding Domain-caffeine nanobody fusion protein BBa_K3425100 in backbone pSB3C11 (BBa_K3425001) with promoter J23108 and RBS B0034.  
  
 
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Latest revision as of 01:44, 28 October 2020


DBD-CaFF construct (pSB3C11-J23108)

BBa K3425105 is a device expressing the DNA Binding Domain-caffeine nanobody fusion protein BBa_K3425100 in backbone pSB3C11 (BBa_K3425001) with promoter J23108 and RBS B0034.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2713
    Illegal NheI site found at 1399
    Illegal NheI site found at 2736
    Illegal NheI site found at 2759
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2713
    Illegal BglII site found at 2832
    Illegal BglII site found at 3706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1313
    Illegal AgeI site found at 3398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 6