Difference between revisions of "Part:BBa K133016"

Latest revision as of 05:04, 30 October 2008

CF

CF – Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production..

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1207
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 764
Illegal BamHI site found at 1275
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 301
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K133016 short</partinfo>
-Whole chimeric protein
+CF – Chimeric fusion protein, which retains the central, most antigenic segment of <i>H. pylori</i> (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from <i>E. coli</i>, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of ''E. coli'' with that of ''H. pylori'' enables activation of innate immunity - a characteristic that flagellin of ''H. pylori'' alone does not posess - while presenting the antigen of ''H. pylori''  for antibody production..
 <!-- Add more about the biology of this part here