Difference between revisions of "Part:BBa K3504005"

Latest revision as of 19:30, 26 October 2020

Malat1 triple helix

NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.

Part Description

Malat1 is an RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA that has been used in several synthetic biology studies to stabilize engineered transcripts.

Usage

MALAT1 is a sequence that would stabilize the transcript and allow for translation after removal of special degradation tags. A RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA has been utilized in a few manufactured science studies for stabilization of engineered transcripts. Overall this is an ON-switch that displays a promising scope of 166-fold, which is unique as an RNA ON-switch motif for transgene regulation.(1)

References

1-DiAndreth, B., Wauford, N., Hu, E., Palacios, S., & Weiss, R. (2019, January 01). PERSIST: A programmable RNA regulation platform using CRISPR endoRNases. Retrieved October 26, 2020, from https://www.biorxiv.org/content/10.1101/2019.12.15.867150v1.full Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 38
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 38
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 38
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 38
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3504005 short</partinfo>
+<p style="color:red">NOTICE: Parts  in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.</p><br/>
 ==Part Description==
@@ Line 9: / Line 9: @@
 ==Usage==
 MALAT1 is a sequence that would stabilize the transcript and allow for translation after removal of special degradation tags.
-A RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA has been utilized in a few manufactured science studies for stabilization of engineered transcripts. Overall this is an ON-switch that displays a promising scope of 166-fold, which is unique as an RNA ON-switch motif for transgene regulation.
+A RNA triple helix (triplex) structure from the MALAT1 long noncoding RNA has been utilized in a few manufactured science studies for stabilization of engineered transcripts. Overall this is an ON-switch that displays a promising scope of 166-fold, which is unique as an RNA ON-switch motif for transgene regulation.(1)
+==References==
+-DiAndreth, B., Wauford, N., Hu, E., Palacios, S., &amp; Weiss, R. (2019, January 01). PERSIST: A programmable RNA regulation platform using CRISPR endoRNases. Retrieved October 26, 2020, from https://www.biorxiv.org/content/10.1101/2019.12.15.867150v1.full
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>