Difference between revisions of "Part:BBa K3381004"

Latest revision as of 01:33, 24 October 2020

FutA2-CBM2a

FutA2-CBM2a is a fusion protein consisting of FutA2 (BBa_K3381001), an iron-binding domain, as well as CBM2a, a cellulose-binding domain. A linker sequence that was found to naturally occur in a CBM2a fusion was used as a linker domain between FutA2 and CBM2a (Courtade, 2018). The CBM2a and flexible linker sequence was submitted as part BBa_K3381007. Additionally, a sequence for a His-tag as well as a TEV recognition sequence was added to the N-terminus for ease of purification (by immobilized-metal affinity chromatography [IMAC]).

Fusion proteins containing cellulose-binding modules (CBMs) have readily been used in industrial purification processes, where the CBMs act as affinity tags. Strikingly enough, waste processing seems to be an application that has not been as fervently explored (Zhou, 2020). Furthermore, current waste processing methods fail to provide a way to recover metal ions once extracted, leaving the playing field open to technologies that make metal recovery possible.

FutA2-CBM2a is a fusion protein that allows for extraction and recovery of iron(III) from aqueous waste. It binds irreversibly to cellulose, and can also bind Fe(III) (Koropatkin, 2007). This makes it appropriate for affinity chromatography or related uses.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1442
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3381004 short</partinfo>
-FutA2-CBM2a is a fusion protein consisting of FutA2, an iron-binding domain, as well as CBM2a, a cellulose-binding domain. A linker sequence that was found to naturally occur in a CBM2a fusion was used as a linker domain between FutA2 and CBM2a (Courtade, 2018). The CBM2a and flexible linker sequence was submitted as part [https://parts.igem.org/Part:BBa_K3381007 BBa_K3381007]. Additionally, a sequence for a His-tag as well as a TEV recognition sequence was added to the N-terminus for ease of purification (by immobilized-metal affinity chromatography [IMAC]).
+FutA2-CBM2a is a fusion protein consisting of FutA2 ([https://parts.igem.org/Part:BBa_K3381001 BBa_K3381001]), an iron-binding domain, as well as CBM2a, a cellulose-binding domain. A linker sequence that was found to naturally occur in a CBM2a fusion was used as a linker domain between FutA2 and CBM2a (Courtade, 2018). The CBM2a and flexible linker sequence was submitted as part [https://parts.igem.org/Part:BBa_K3381007 BBa_K3381007]. Additionally, a sequence for a His-tag as well as a TEV recognition sequence was added to the N-terminus for ease of purification (by immobilized-metal affinity chromatography [IMAC]).
 Fusion proteins containing cellulose-binding modules (CBMs) have readily been used in industrial purification processes, where the CBMs act as affinity tags. Strikingly enough, waste processing seems to be an application that has not been as fervently explored (Zhou, 2020). Furthermore, current waste processing methods fail to provide a way to recover metal ions once extracted, leaving the playing field open to technologies that make metal recovery possible.
 FutA2-CBM2a is a fusion protein that allows for extraction and recovery of iron(III) from aqueous waste. It binds irreversibly to cellulose, and can also bind Fe(III) (Koropatkin, 2007). This makes it appropriate for affinity chromatography or related uses.