Difference between revisions of "Part:BBa K3463024:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Characterization of BBa_K3463024=== |
| + | we transformed BL21 strains and from an overnight culture, we inoculated them at 0.5 of OD 600nm. Triplicates of 200 µl were performed for fluorescence hourly monitoring in 96-well plates using FluoStar machines during 10 hour . Optical density was also measured. To compare the plsr_ high expression’s dynamic we used BL21 WT and a constitutive promoter (J23100) as control. LB was used as blank. | ||
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| + | [[Image:exp delivery sys.png|600px|thumb|center| mCherry fluorescence expression reported at bacterial optical density over time.To represent the two curves, we used two gradations: the one on the left represents the expression of the plsr_low and the one on the right represents the J23100 promoter. To highlight the actual production of mCherry we subtracted the basal fluorescence value and divided by the optical density in order to normalize the fluorescence values. ]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 17:10, 26 October 2020
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how you used this part and how it worked out.
Characterization of BBa_K3463024
we transformed BL21 strains and from an overnight culture, we inoculated them at 0.5 of OD 600nm. Triplicates of 200 µl were performed for fluorescence hourly monitoring in 96-well plates using FluoStar machines during 10 hour . Optical density was also measured. To compare the plsr_ high expression’s dynamic we used BL21 WT and a constitutive promoter (J23100) as control. LB was used as blank.
mCherry fluorescence expression reported at bacterial optical density over time.To represent the two curves, we used two gradations: the one on the left represents the expression of the plsr_low and the one on the right represents the J23100 promoter. To highlight the actual production of mCherry we subtracted the basal fluorescence value and divided by the optical density in order to normalize the fluorescence values.
User Reviews
UNIQ7e6e63f217d8b6cb-partinfo-00000000-QINU UNIQ7e6e63f217d8b6cb-partinfo-00000001-QINU