Difference between revisions of "Part:BBa K3697002:Design"
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===Source=== | ===Source=== | ||
| − | This part was derived from the genome of Bacillus Subtilis 168 and initially used in Altenbuchner, 2015 | + | This part was derived from the genome of Bacillus Subtilis 168 and initially used in Altenbuchner, 2015 [1] |
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| + | [1] Wenzel, M. and Altenbuchner, J. (2015) Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate‐dependent phosphotransferase system. Microbiology (United Kingdom), 161(10), 1942–1949. | ||
===References=== | ===References=== | ||
Latest revision as of 00:28, 24 October 2020
manP expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 129
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 722
Illegal AgeI site found at 372
Illegal AgeI site found at 466
Illegal AgeI site found at 2132 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 272
Illegal SapI site found at 1294
Design Notes
When designing this part, we made to sure not to subdivide the expression cassette as we wanted to insure that manP was produced at as close to a natural level as possible in the strain we used.
Source
This part was derived from the genome of Bacillus Subtilis 168 and initially used in Altenbuchner, 2015 [1]
[1] Wenzel, M. and Altenbuchner, J. (2015) Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate‐dependent phosphotransferase system. Microbiology (United Kingdom), 161(10), 1942–1949.