Difference between revisions of "Part:BBa K3544001:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
work together with BcABA1,BcABA3 and BcABA4 for heterologous expression of ABA in our project
+
An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into 1',4'-trans-dihydroxy-α-ionylideneacetic acid.
  
  
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===Source===
 
===Source===
  
−
Botrytis Cinerea
+
The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in S.c and synthesized from Sangon
  
 
===References===
 
===References===
 +
 +
Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.

Latest revision as of 13:29, 27 October 2020


Coding sequence of BcABA2, a cytochrome P450s belong to biosynthetic genecluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1423
    Illegal PstI site found at 956
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 956
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1423
    Illegal PstI site found at 956
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1423
    Illegal PstI site found at 956
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into 1',4'-trans-dihydroxy-α-ionylideneacetic acid.



Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in S.c and synthesized from Sangon

References

Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.