Difference between revisions of "Part:BBa K3331018"

 
 
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<partinfo>BBa_K3331018 parameters</partinfo>
 
<partinfo>BBa_K3331018 parameters</partinfo>
 
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===Characterization===
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We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.
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<div>[[File:T--XJTU-China--Implementation03.png |700px|thumb|center|<b>Figure 1:</b>Experimental medium. 6-1 is blank control ,6-4 is experimental group.
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The protocol of the experiment is list below.]]</div>

Latest revision as of 20:33, 27 October 2020


E.coli BAD +MazF

This is a suicide switch controlled by an arabinose operon. When arabinose is present, the bacteria activate the suicide switch and die.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2001
    Illegal BamHI site found at 1548
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We transferred the plasmid pBAD-MazF into E.coli DH5α. The bacteria in the experimental group were cultured in the liquid LB-ampicillin medium supplemented with L-arabinose. The concentration of L-arabinose is 1.5μM/L. The bacteria liquid was then diluted and spread on solid medium. 24 hours later, no obvious colony was observed in the group with L-arabinose, but the bacteria in the blank control group grew normally. It is proved that the growth of bacteria is inhibited in the case of MazF expression.

Figure 1:Experimental medium. 6-1 is blank control ,6-4 is experimental group. The protocol of the experiment is list below.