Difference between revisions of "Part:BBa K3482042"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3482019 short</partinfo>
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<partinfo>BBa_K3482042 short</partinfo>
  
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The CcdA protein gives an immunity against the CcdB toxin by strong complex CcdA-CcdB and therefore prenvent the CcdB toxin to interact with the DNA gyrase.
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Azurin protein fused with NSP4 secretion signial peptide and a 8His secretion tag.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.
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We used this part with a tag for protein expression and purification
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3482019 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3482042 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3482019 parameters</partinfo>
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<partinfo>BBa_K3482042 parameters</partinfo>
 
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Latest revision as of 17:16, 26 October 2020


azurin with NSP4 secretion and 8His tag

Azurin protein fused with NSP4 secretion signial peptide and a 8His secretion tag.

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part with a tag for protein expression and purification


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]