Difference between revisions of "Part:BBa K3482007"
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<partinfo>BBa_K3482007 short</partinfo> | <partinfo>BBa_K3482007 short</partinfo> | ||
| − | This 8x Histidine tag can be used | + | This 8x Histidine tag can be used for the affinity purification of a tagged protein. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The His tag has been used to purify the azurin secreted outside of the E.coli | + | The His tag has been used to purify the azurin secreted outside of the <i>E. coli Nissle 1917</i> cells. |
Latest revision as of 19:20, 27 October 2020
8x Histidine tag for protein purification
This 8x Histidine tag can be used for the affinity purification of a tagged protein.
Usage and Biology
The His tag has been used to purify the azurin secreted outside of the E. coli Nissle 1917 cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]