Difference between revisions of "Part:BBa K3328034"
 
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triggerB of XOR1 logic gate to perform the truth table
 
triggerB of XOR1 logic gate to perform the truth table
 
===Design===
 
===Design===
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The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.
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The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state.
  
 
https://2020.igem.org/wiki/images/thumb/9/99/T--OUC-China--design_lunbo_xor.jpg/799px-T--OUC-China--design_lunbo_xor.jpg
 
https://2020.igem.org/wiki/images/thumb/9/99/T--OUC-China--design_lunbo_xor.jpg/799px-T--OUC-China--design_lunbo_xor.jpg
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===Result===
 
===Result===
 
https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg
 
https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg
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The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
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The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
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Latest revision as of 11:44, 27 October 2020


triggerB of XOR gate (XOR1)

triggerB of XOR1 logic gate to perform the truth table

Design

The XOR is inspired by the NIMPLY gate, consisting of a toehold switch and two triggers. The trigger’s core sequence is the same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. Moreover, when input these two triggers simultaneously, they can pair together and form a ring in the middle. As a result, the switch will still be in the OFF state.

799px-T--OUC-China--design_lunbo_xor.jpg

Result

800px-T--OUC-China--part_XOR1.jpg The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]