Difference between revisions of "Part:BBa K3365053:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR. | ||
===Source=== | ===Source=== | ||
| − | We | + | We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China. |
===References=== | ===References=== | ||
Latest revision as of 12:56, 27 October 2020
RFP with double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 700
Illegal XhoI site found at 691 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Be careful especially when design primers. Due to the complex secondary structure of double terminator, we put a small nonfunctional sequence downstream of the double terminator as linker for the convenience of PCR.
Source
We amplify this part on plasmid pE1a-RFP, which is offered by team Tongji_China.