Difference between revisions of "Part:BBa K3482005"
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This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell. | This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell. | ||
| + | ===Usage and Biology=== | ||
| + | |||
| + | The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in <i>E. coli</i>. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our <i>E. coli Nissle 1917</i> strain. | ||
Latest revision as of 17:42, 27 October 2020
NSP4 secretion signal peptide
This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell.
Usage and Biology
The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in E. coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our E. coli Nissle 1917 strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
- 1000COMPATIBLE WITH RFC[1000]