Difference between revisions of "Part:BBa K3657050"

 
 
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<partinfo>BBa_K3657050 short</partinfo>
 
<partinfo>BBa_K3657050 short</partinfo>
  
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Single-guide RNA for use with the catalytic core of a Tetrahymena thermophila trans-splicing ribozyme.
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Splicing results in the replacement of the 3' intron of the target sequence with mScarlet.
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For more details see the design section of this part.
  
 
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Latest revision as of 03:44, 28 October 2020


Double Split-Tetrahymena Ribozyme 3'Guide + mScarlet

Single-guide RNA for use with the catalytic core of a Tetrahymena thermophila trans-splicing ribozyme. Splicing results in the replacement of the 3' intron of the target sequence with mScarlet. For more details see the design section of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 540
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]