Difference between revisions of "Part:BBa K136064"
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It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ). | It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ). | ||
− | <br>This construction may allow ,with the add of a reporter tag, to see some oscillations | + | <br>This construction may allow, with the add of a reporter tag, to see some oscillations |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 02:10, 29 October 2008
fliL promoter + WT RBS ( K136009) - EnvZ*
EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.
fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).
Its promoter is consitutively repressed.
It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
This construction may allow, with the add of a reporter tag, to see some oscillations
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 476
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 476
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 476
Illegal BglII site found at 396 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 476
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 476
- 1000COMPATIBLE WITH RFC[1000]