Difference between revisions of "Part:BBa K3447007"
 
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<partinfo>BBa_K3447007 short</partinfo>
 
<partinfo>BBa_K3447007 short</partinfo>
  
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P<sub>BAD</sub> promoter is an inducible promoter, P<sub>BAD</sub> contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only <i>araC</i> and P<sub>BAD</sub>.<br>
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Axh43 gene, also called xynD, is derived from <i>Bacillus subtilis</i> 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.
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===Usage and Biology===
 
===Usage and Biology===
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In the arabinose operon, it is regulated by the AraC operon and activates the expression of downstream arabinose metabolism genes in the presence of arabinose inducers.<br>
 
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The L-Arabinose operon is naturally present in <i>E. coli</i>.The regulatory protein AraC ACTS as a dimer. The operon contains two cis-type control elements: The P<sub>C</sub> promoter for the synthesis of AraC and the P<sub>BAD</sub> for the synthesis of the enzyme needed for the catabolism of L-Arabinose. P<sub>BAD</sub> contains three half sites, each of which binds to a subunit of ARAC-O2, I1, and I2. The O1 site is composed of O1L and O1R half sites that bind two subunits. Half of O2 is in the araC coding region.
 
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We connected the target genes downstream of P<sub>BAD</sub> to constitute the effect part of arabinose sensing system, and constructed the whole arabinose sensing system on the same plasmid with P<sub>C</sub> and araC.
 
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Arabinose induces endotoxin expression: <partinfo>BBa_K3447000</partinfo>
 
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Arabinose induces green fluorescent protein expression: (链接a01+c09)
 
  
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In our project, we use this part together with xynA gene  <partinfo>BBa_K1175005</Partinfo> to hydrolyze xylan and produce arabinose. For more information, please check out our part <partinfo>BBa_K3447102</Partinfo>.
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==<b>Design</b>==
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===Design Notes===
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There is no need to pay attention.<br>
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===Source===
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This gene is derived from <i>Bacillus subtilis</i> 168, we get the genomic sequence by NCBI.<br>
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We ordered the part DNA from a synthesis company.<br>
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3447007 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 10:26, 27 October 2020


xynD, which produces xylanase

Axh43 gene, also called xynD, is derived from Bacillus subtilis 168 and belongs to the GH43 hydrolase family. It can specifically hydrolyze the Arabinose residues on the O-2 xylan or o-3 xylan, and assist the hydrolysis of the xylan.


Usage and Biology

In our project, we use this part together with xynA gene BBa_K1175005 to hydrolyze xylan and produce arabinose. For more information, please check out our part BBa_K3447102.

Design

Design Notes

There is no need to pay attention.

Source

This gene is derived from Bacillus subtilis 168, we get the genomic sequence by NCBI.
We ordered the part DNA from a synthesis company.