Difference between revisions of "Part:BBa K3605004"

 
 
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Endo-β-1,4-glucanase(CMCax) is from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for Bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited.
 
Endo-β-1,4-glucanase(CMCax) is from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for Bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited.
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This part is Endo-β-1,4-glucanase(CMCax) with 1080bp, from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited. This gene was amplified using PCR method, and then inserted into the vector pSB1C3. The identification result is showed in Fig.1.
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[[File:K3605004-1.jpg|center]]
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Fig.1. The result of CMCax gene cloning.
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M: Marker; 1: PCR result of CMCax; 2: Digestion of pSB1C3 containing CMCax.
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Latest revision as of 16:46, 27 October 2020


Endo-β-1,4-glucanase(CMCax) from Gluconacetobacter xylinus.

Endo-β-1,4-glucanase(CMCax) is from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for Bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited.

This part is Endo-β-1,4-glucanase(CMCax) with 1080bp, from Gluconacetobacter xylinus. It can hydrolyze the glucan chain and help straighten out the glucan, which is important for bacterial cellulose. When antibodies to recombinant CMCax are added to the culture medium, the formation of cellulose fibre is severely inhibited. This gene was amplified using PCR method, and then inserted into the vector pSB1C3. The identification result is showed in Fig.1.

K3605004-1.jpg

Fig.1. The result of CMCax gene cloning. M: Marker; 1: PCR result of CMCax; 2: Digestion of pSB1C3 containing CMCax.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]