Difference between revisions of "Part:BBa K3657037"

 
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This part is an RNA containing binding sites for Pumby protein binding AUAGAU (<a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>) and Pumby protein binding AUGGUU (<a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>).
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===Usage and Biology===
 
===Usage and Biology===
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Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide.
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Therefore the protein can be designed to bind a specific RNA sequence.</p>
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<p>It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: <a href="https://parts.igem.org/Part:BBa_K3657034">BBa_K3657034</a>, <a href="https://parts.igem.org/Part:BBa_K3657035">BBa_K3657035.</a>
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<p>Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020</a></p>
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<img style="width:75%" src="https://static.igem.org/mediawiki/parts/d/de/T--Heidelberg--Parts-BBa_K3657037.jpg">
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<p><b>Figure 1:</b> Secondary structure of the part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657037 SequenceAndFeatures</partinfo>

Latest revision as of 03:51, 28 October 2020


RNA Linker binding Pumby AUAGAU and Pumby AUGGUU
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (BBa_K3657004) and Pumby protein binding AUGGUU (BBa_K3657005).

Usage and Biology

Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. Therefore the protein can be designed to bind a specific RNA sequence.

It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: BBa_K3657034, BBa_K3657035.

Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: iGEM Heidelberg 2020

Figure 1: Secondary structure of the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]