Difference between revisions of "Part:BBa K3605002"

 
 
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<partinfo>BBa_K3605002 short</partinfo>
  
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This part contains two enzymes (2277bp), which is a polycistron in original organism. One of them is phosphoglucomutase (PGM), it can isomerize the glucose-6-phosphate (G-6-P) to glucose-1-phosphate (G-1-P), which is the second reaction for bacterial cellulose production from glucose. Another enzyme is UDP-glucose pyrophosphorylase (UGPase), it can catalyze the glucose-1-phosphate (G-1-P) to react with UTP, forming uridine-5’-phosphate-α-D-glucose (UDPG), which is the third reaction for Bacterial cellulose production from glucose.
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[[File:K3605002-1.jpg|center]]
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These two enzymes were also amplified using PCR method, and inserted into the vector pSB1C3. Fig.1 shows the identification result.
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[[File:K3605002-2.jpg|center]]
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Fig.1. The result of PGM and UGPase gene cloning.
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M: Marker; 1: PCR result of PGM and UGPase; 2: Digestion of pSB1C3 containing PGM and UGPase genes.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3605002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3605002 parameters</partinfo>
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Latest revision as of 17:14, 27 October 2020

Phosphoglucomutase (PGM) and UDP-glucose pyrophosphorylase (UGPase) from Gluconacetobacter xylinus.

This part contains two enzymes (2277bp), which is a polycistron in original organism. One of them is phosphoglucomutase (PGM), it can isomerize the glucose-6-phosphate (G-6-P) to glucose-1-phosphate (G-1-P), which is the second reaction for bacterial cellulose production from glucose. Another enzyme is UDP-glucose pyrophosphorylase (UGPase), it can catalyze the glucose-1-phosphate (G-1-P) to react with UTP, forming uridine-5’-phosphate-α-D-glucose (UDPG), which is the third reaction for Bacterial cellulose production from glucose.

K3605002-1.jpg



These two enzymes were also amplified using PCR method, and inserted into the vector pSB1C3. Fig.1 shows the identification result.

K3605002-2.jpg

Fig.1. The result of PGM and UGPase gene cloning. M: Marker; 1: PCR result of PGM and UGPase; 2: Digestion of pSB1C3 containing PGM and UGPase genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757