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This is a fusion protein containing AUAGAU binding Pumby Protein (<a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>) and N-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657027">BBa_K3657027</a>).
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===Usage and Biology===
 
===Usage and Biology===
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<p>AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). Pumby Proteins can be designed to bind a specific RNA sequence.</p>
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<p>N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657028">BBa_K3657028</a>).</p>
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<p>This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: <a href="https://parts.igem.org/Part:BBa_K3657036">BBa_K3657036</a>. The part with the C-terminal of the Split-GFP planned for the experiment: <a href="https://parts.igem.org/Part:BBa_K3657034">BBa_K3657034</a>. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p>
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<p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657033 SequenceAndFeatures</partinfo>

Latest revision as of 02:22, 28 October 2020


RNA AUAGAU binding Pumby Protein coupled with N-terminal GFP'

This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and N-terminal Split-GFP (BBa_K3657027).

Usage and Biology

AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.

N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (BBa_K3657028).


This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the C-terminal of the Split-GFP planned for the experiment: BBa_K3657034. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 679
    Illegal XhoI site found at 719
    Illegal XhoI site found at 944
    Illegal XhoI site found at 1129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]