Difference between revisions of "Part:BBa K3407006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Design | + | This biobrick represents the DNA template for the transcription of a 65 nt shRNA, excluding the promoter. Trancription was performed with <html><a id="6" href="https://parts.igem.org/Part:BBa_K3407022" target="_blank"><b>BBa_K3407022</b></a></html>. The dsRNA sequence was taken from pUC57-OriLR-deGFP plasmid (BBa_K3407006). It corresponds to 27nt from the eGFP gene (nt 78 to 105), and is the one recognised and processed by Dicer to form siRNA. The shRNA has a single-stranded RNA (ssRNA) loop region designed to be recognised by Fox-1 RBD (<html><a id="6" href="https://parts.igem.org/Part:BBa_K3407004" target="_blank"><b>BBa_K3407004</b></a></html>), containing its “<html><u>UGCAUGU</u><html>” RNA target sequence <html><a href="#1">[1]</a></html>. The dsRNA region contains a GG overhang that would represent the overhang left when mini-3 (<html><a id="6" href="https://parts.igem.org/Part:BBa_K3407002" target="_blank"><b>BBa_K3407002</b></a></html>) cleaves a tshRNA (See <html><a id="6" href="https://2020.igem.org/Team:TUDelft" target="_blank"><b>iGEM TU Delft 2020</b></a></html>, Design - Future perspectives), and that represents a good substrate to process the shRNA by Dicer into a siRNA. |
+ | ===References=== | ||
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+ | <a id="1" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1356361/" target="_blank"> | ||
+ | Auweter, S., Fasan, R., Reymond, L., Underwood, J., Black, D., Pitsch, S. and Allain, F., 2020. Molecular Basis Of RNA Recognition By The Human Alternative Splicing Factor Fox-1.</a> | ||
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Latest revision as of 10:31, 27 October 2020
Short hairpin RNA (shRNA): a dmDicer-2 substrate and potential trigger of RNAi.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick represents the DNA template for the transcription of a 65 nt shRNA, excluding the promoter. Trancription was performed with BBa_K3407022. The dsRNA sequence was taken from pUC57-OriLR-deGFP plasmid (BBa_K3407006). It corresponds to 27nt from the eGFP gene (nt 78 to 105), and is the one recognised and processed by Dicer to form siRNA. The shRNA has a single-stranded RNA (ssRNA) loop region designed to be recognised by Fox-1 RBD (BBa_K3407004), containing its “UGCAUGU” RNA target sequence [1]. The dsRNA region contains a GG overhang that would represent the overhang left when mini-3 (BBa_K3407002) cleaves a tshRNA (See iGEM TU Delft 2020, Design - Future perspectives), and that represents a good substrate to process the shRNA by Dicer into a siRNA.
References