Difference between revisions of "Part:BBa K131019"

Latest revision as of 01:19, 29 October 2008

LuxO inducible RFP

p_qrr4+Q04510+I3502

Usage and Biology

This is our empty response circuit for the quorum signaling molecule, AI-2. This was to go into our champion cell, along with the sensing circuit for AI-2, which contains, under regulation, the protein necessary to activate this circuit, LuxO (BBa_K131016). When LuxO is dephosphorylated, which occurs when AI-2 is present, transcription stops from BBa_K131017, leading to no more cl lam, and hence no more repression on the cl lam promoter. Thus, RFP is produced only when AI-2 is sensed. This construct has no terminators, as our champion cell is intended to produce both RFP and colicin E3 (an antibiotic), however this intermediate allows any DNA to be expressed along with RFP, under control of AI-2.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1851
Illegal AgeI site found at 1963
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-This is our empty response circuit for the quorum signaling molecule, AI-2.  This was to go into our champion cell, along with the sensing circuit for AI-2, which contains, under regulation, the protein necessary to activate this circuit, LuxO (<bbpart>BBa_K131016</bbpart>).  When LuxO is dephosphorylated, which occurs when AI-2 is present, transcription stops from <bbpart>BBa_K131017</bbpart>, leading to no more cl lam, and hence no more repression on the cl lam promoter.  Thus, RFP is produced only when AI-2 is sensed.  This construct has no terminators, as our champion cell is intended to produce both RFP and colicin E3 (an antibiotic).
+This is our empty response circuit for the quorum signaling molecule, AI-2.  This was to go into our champion cell, along with the sensing circuit for AI-2, which contains, under regulation, the protein necessary to activate this circuit, LuxO (<bbpart>BBa_K131016</bbpart>).  When LuxO is dephosphorylated, which occurs when AI-2 is present, transcription stops from <bbpart>BBa_K131017</bbpart>, leading to no more cl lam, and hence no more repression on the cl lam promoter.  Thus, RFP is produced only when AI-2 is sensed.
+This construct has no terminators, as our champion cell is intended to produce both RFP and colicin E3 (an antibiotic), however this intermediate allows any DNA to be expressed along with RFP, under control of AI-2.
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