Difference between revisions of "Part:BBa K3657013:Design"

 
 
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===Design Notes===
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PPR Proteins binding a specific RNA sequence can be assebled, usin the base-specific domains:
  
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<br>
  
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<p> Adenine:<a href="https://parts.igem.org/Part:BBa_K3657006">BBa_K3657006</a><p>
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Guanine:<a href="https://parts.igem.org/Part:BBa_K3657007">BBa_K3657007</a><p>
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Uracil:<a href="https://parts.igem.org/Part:BBa_K3657008">BBa_K3657008</a><p>
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Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657009">BBa_K3657009</a><p>
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For more information on the design, visit <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR#pumby">iGEM Heidelberg 2020</a></p>
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===Source===
 
===Source===
  
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<i>Arabidopsis thaliana</i>
  
 
===References===
 
===References===
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S. Aubourg and N. Boudet and M. Kreis and A. Lecharny (2004) In Arabidopsis thaliana, 1% of the genome codes for a novel protein family unique to plants.Plant Molecular Biology 42, 603-613
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S. Manna (2015) An overview of pentatricopeptide repeat proteins and their applications..Biochimie 113, 93-9
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A. Barkan and Margarita Rojas and S. Fujii and Aaron Yap and Y. S. Chong and C. Bond and I. Small (2012) A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins.PLoS Genetics 8

Latest revision as of 01:55, 28 October 2020


RNA binding Pentatricopeptide Repeat Protein - Motif GUAGAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PPR Proteins binding a specific RNA sequence can be assebled, usin the base-specific domains:

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

For more information on the design, visit iGEM Heidelberg 2020

Source

Arabidopsis thaliana

References

S. Aubourg and N. Boudet and M. Kreis and A. Lecharny (2004) In Arabidopsis thaliana, 1% of the genome codes for a novel protein family unique to plants.Plant Molecular Biology 42, 603-613

S. Manna (2015) An overview of pentatricopeptide repeat proteins and their applications..Biochimie 113, 93-9

A. Barkan and Margarita Rojas and S. Fujii and Aaron Yap and Y. S. Chong and C. Bond and I. Small (2012) A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins.PLoS Genetics 8