Difference between revisions of "Part:BBa K3512000"
 
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<partinfo>BBa_K3512000 short</partinfo>
 
<partinfo>BBa_K3512000 short</partinfo>
  
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This is the anti-invertase or invertase inhibitor from Sugarcane (<em>Saccharum officinarum</em>)
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This is the anti-invertase or invertase inhibitor from Sugarcane (<em>Saccharum officinarum</em>). It is part of the Fructose Regulated Anti-invertase Construct (BBa_K3512012).
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In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play a key role in post-translation regulation of plant invertases through which sucrose hydrolysis is controlled.
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<h2>Homology Modelling</h2>
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Homology modelling was used to create the structure of sugarcane invertase using SwissModel.
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[[Image:BBa_K3512000_Anti.png|400px|thumb|center|Homology Modelling of Sugarcane Invertase Inhibitor]]
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<h3>Ramachandran Plot</h3>
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A Ramachandran plot is a way to visualize energetically favoured regions for backbone dihedral angles against amino acid residues in protein structure.
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[[Image:BBa_K3512000_RP.png|400px|thumb|center| Ramachandran Plot ]]
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97.48% is Ramachandran Favoured. ALA120, PHE148 have C-Beta Deviations.
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<h3>Quality Estimation</h3>
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The comparison with Non-Redundant Set of PDB Structures helps us derive a quality estimate for our model via QMEAN4.
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The model had a GMQE of 1.24
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[[Image:BBa_K3512000_QE.png|400px|thumb|center| Quality Estimation]]
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<h2>Invertase Anti-invertase Docking</h2>
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The following image illustrates the docking of sugarcane invertase with sugarcane invertase inhibitor. The final energy of binding is -667.5J. ClusPro docking server was used to perform the protein-protein docking.
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[[Image:BBa_K3512000_IA.png|400px|thumb|center| Protein-Protein Docking]]
  
 
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Latest revision as of 20:39, 21 October 2020


Sugarcane Invertase Inhibitor

This is the anti-invertase or invertase inhibitor from Sugarcane (Saccharum officinarum). It is part of the Fructose Regulated Anti-invertase Construct (BBa_K3512012).

In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play a key role in post-translation regulation of plant invertases through which sucrose hydrolysis is controlled.

Homology Modelling

Homology modelling was used to create the structure of sugarcane invertase using SwissModel.

Homology Modelling of Sugarcane Invertase Inhibitor

Ramachandran Plot

A Ramachandran plot is a way to visualize energetically favoured regions for backbone dihedral angles against amino acid residues in protein structure.

Ramachandran Plot
97.48% is Ramachandran Favoured. ALA120, PHE148 have C-Beta Deviations.

Quality Estimation

The comparison with Non-Redundant Set of PDB Structures helps us derive a quality estimate for our model via QMEAN4. The model had a GMQE of 1.24

Quality Estimation

Invertase Anti-invertase Docking

The following image illustrates the docking of sugarcane invertase with sugarcane invertase inhibitor. The final energy of binding is -667.5J. ClusPro docking server was used to perform the protein-protein docking.

Protein-Protein Docking

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]