Difference between revisions of "Part:BBa K3381002"

 
 
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<partinfo>BBa_K3381002 short</partinfo>
  
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Mst-CopC is a copper binding protein with a single binding site that preferentially binds Cu(II). Published binding affinities for Mst-CopC did not include the variant present in Methylosinus trichosporium; however, our molecular dynamics simulation showed that it had a total interaction energy of -540.64 KJ/mol. This was done with a 10 nanosecond simulation using GROMACS and the Charmm36 force field (Vanommeslaeghe, 2010) (see our [https://2020.igem.org/Team:Waterloo/Engineering engineering] and [https://2020.igem.org/Team:Waterloo/Model model] pages for more information). Mst-CopC binds Cu(II) with a “distorted square pyramidal geometry with H23, H107, D105, and the amino terminus comprising the planar ligands and a water molecule serving as the axial ligand” (Lawton, Kenney, Hurley, & Rosenzweig, 2016).
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3381002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3381002 parameters</partinfo>
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Latest revision as of 01:29, 24 October 2020

Mst-CopC (copper-binding)

Mst-CopC is a copper binding protein with a single binding site that preferentially binds Cu(II). Published binding affinities for Mst-CopC did not include the variant present in Methylosinus trichosporium; however, our molecular dynamics simulation showed that it had a total interaction energy of -540.64 KJ/mol. This was done with a 10 nanosecond simulation using GROMACS and the Charmm36 force field (Vanommeslaeghe, 2010) (see our engineering and model pages for more information). Mst-CopC binds Cu(II) with a “distorted square pyramidal geometry with H23, H107, D105, and the amino terminus comprising the planar ligands and a water molecule serving as the axial ligand” (Lawton, Kenney, Hurley, & Rosenzweig, 2016).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]