Difference between revisions of "Part:BBa K142047:Design"
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===Source=== | ===Source=== | ||
| − | Assembled from parts K142046 and I739001 by standard cloning techniques. | + | Assembled from parts K142046 and I739001 by standard cloning techniques. Please see [http://2008.igem.org/Team:ETH_Zurich/Wetlab/Switch_Circuit ETH Zurich Wiki] for detailed documentation. |
===References=== | ===References=== | ||
Latest revision as of 23:48, 28 October 2008
tet-controlled LacI generator with constitutive TetR expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1385
Illegal NheI site found at 1408 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1227
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to put the constitutive expression cassette behind the inducible LacI generator to minimze readthrough from the constitutive expression.
Source
Assembled from parts K142046 and I739001 by standard cloning techniques. Please see [http://2008.igem.org/Team:ETH_Zurich/Wetlab/Switch_Circuit ETH Zurich Wiki] for detailed documentation.