Difference between revisions of "Part:BBa K3328053"
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===Design=== | ===Design=== | ||
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation. | OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation. | ||
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| + | https://2020.igem.org/wiki/images/thumb/b/b3/T--OUC-China--design_lunbo_or.jpg/799px-T--OUC-China--design_lunbo_or.jpg | ||
===Result=== | ===Result=== | ||
https://2020.igem.org/wiki/images/thumb/6/6c/T--OUC-China--part_OR.jpg/800px-T--OUC-China--part_OR.jpg | https://2020.igem.org/wiki/images/thumb/6/6c/T--OUC-China--part_OR.jpg/800px-T--OUC-China--part_OR.jpg | ||
| − | The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ | + | The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9). |
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Latest revision as of 12:50, 27 October 2020
switch of OR gate
OR switch
Design
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.
Result
The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]