Difference between revisions of "Part:BBa K3328027"
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===Design===
 
===Design===
 
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.  
 
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.  
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https://2020.igem.org/wiki/images/thumb/b/b3/T--OUC-China--design_lunbo_or.jpg/799px-T--OUC-China--design_lunbo_or.jpg
  
 
===Result===
 
===Result===
 
https://2020.igem.org/wiki/images/thumb/6/6c/T--OUC-China--part_OR.jpg/800px-T--OUC-China--part_OR.jpg
 
https://2020.igem.org/wiki/images/thumb/6/6c/T--OUC-China--part_OR.jpg/800px-T--OUC-China--part_OR.jpg
  
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The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
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The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:16, 27 October 2020


switch of OR gate

realize the boolean calculation of OR

Design

OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.

799px-T--OUC-China--design_lunbo_or.jpg

Result

800px-T--OUC-China--part_OR.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 819