Difference between revisions of "Part:BBa K2904111"

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This promoter is induced by aTc. It was used by 2019 OUC-China team.  
 
This promoter is induced by aTc. It was used by 2019 OUC-China team.  
  
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===2020 OUC-China===
 
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It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.
 
  
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==<strong>OUC-China 2020’s characterization</strong>==
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===Design===
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It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.
 
===Result===
 
===Result===
 
https://2020.igem.org/wiki/images/thumb/8/86/T--OUC-China--result_fig1A_pTet.jpg/800px-T--OUC-China--result_fig1A_pTet.jpg
 
https://2020.igem.org/wiki/images/thumb/8/86/T--OUC-China--result_fig1A_pTet.jpg/800px-T--OUC-China--result_fig1A_pTet.jpg
  
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The Tet promoter fluorescence data in BL21 (DE3) at different aTc concentrations (0mg/ml, 0.01mg/ml, 0.1mg/ml, 0.25mg/ml, 0.5mg/ml and 1mg/ml). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/ml.
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The Tet promoter fluorescence data in <span style="font-style: italic;">E. coli</span> BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 13:49, 27 October 2020


aTc inducible Tet promoter

This promoter is induced by aTc. It was used by 2019 OUC-China team.


OUC-China 2020’s characterization

Design

It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.

Result

800px-T--OUC-China--result_fig1A_pTet.jpg

The Tet promoter fluorescence data in E. coli BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]