Difference between revisions of "Part:BBa K3328052"

Latest revision as of 12:49, 27 October 2020

triggerB of NIMPLY gate (NIMPLY2)

NIMPLY2 triggerB

Design

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

Result

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 17
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 14
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 17
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 17
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 NIMPLY2 triggerB
+===Design===
+In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
+https://2020.igem.org/wiki/images/thumb/8/87/T--OUC-China--design_lunbo_nimply.jpg/799px-T--OUC-China--design_lunbo_nimply.jpg
+===Result===
+https://2020.igem.org/wiki/images/thumb/1/1f/T--OUC-China--part_NIMPLY2.jpg/800px-T--OUC-China--part_NIMPLY2.jpg
+The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
 <!-- Add more about the biology of this part here