Difference between revisions of "Part:BBa K3351012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3351012 short</partinfo> | <partinfo>BBa_K3351012 short</partinfo> | ||
+ | ===Summary=== | ||
+ | PhaP contains a hydrophobic granule binding domain and a cytosol-facing hydrophilic domain. PhaP-tagged proteins could interact with various types of hydrophobic surfaces. A (Gly4Ser2)2 flexible linker is inserted between PhaP and DCD1L to facilitate attaching of DCD1L on surface and displaying sufficient flexibility of this peptide. | ||
− | + | ===Characterization=== | |
+ | <html> | ||
+ | <img style="display: block; | ||
+ | width: 60%;height: 60%;" src="https://static.igem.org/mediawiki/parts/4/47/T--NWU-CHINA-A--od-7.png"><div>Figure.1 growth curve</div></html> | ||
+ | |||
+ | ===Reference=== | ||
+ | [1] Xue Q, Liu XB, Lao YH, Wu LP, Wang D, Zuo ZQ, Chen JY, Hou J, Bei YY, Wu XF, Leong KW, Xiang H, Han J. Anti-infective biomaterials with surface-decorated tachyplesin I. Biomaterials. 2018 Sep;178:351-362. doi: 10.1016/j.biomaterials.2018.05.008. Epub 2018 May 9. PMID: 29778319. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 10:44, 26 October 2020
HisTag-PhaP-linker-DCD1L-HisTag
Summary
PhaP contains a hydrophobic granule binding domain and a cytosol-facing hydrophilic domain. PhaP-tagged proteins could interact with various types of hydrophobic surfaces. A (Gly4Ser2)2 flexible linker is inserted between PhaP and DCD1L to facilitate attaching of DCD1L on surface and displaying sufficient flexibility of this peptide.
Characterization
Figure.1 growth curve
Reference
[1] Xue Q, Liu XB, Lao YH, Wu LP, Wang D, Zuo ZQ, Chen JY, Hou J, Bei YY, Wu XF, Leong KW, Xiang H, Han J. Anti-infective biomaterials with surface-decorated tachyplesin I. Biomaterials. 2018 Sep;178:351-362. doi: 10.1016/j.biomaterials.2018.05.008. Epub 2018 May 9. PMID: 29778319. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]